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双突触诱发的星形胶质细胞和神经元的反应,在急性海马脑片电生理记录
JoVE Journal
Neuroscience
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JoVE Journal
Neuroscience
Dual Electrophysiological Recordings of Synaptically-evoked Astroglial and Neuronal Responses in Acute Hippocampal Slices
Please note that all translations are automatically generated.
Click here for the English version.
双突触诱发的星形胶质细胞和神经元的反应,在急性海马脑片电生理记录
DOI:
10.3791/4418-v
•
16:38 min
•
November 26, 2012
•
Ulrike Pannasch*
1
,
Jérémie Sibille*
1,2
,
Nathalie Rouach
1
Neuroglial Interactions in Cerebral Physiopathology, Center for Interdisciplinary Research in Biology, CNRS UMR 7241, INSERM U1050
,
Collège de France
,
2
Paris Diderot University
Chapters
00:00
Title
03:58
Dissection
10:23
Dual Recordings of Neuronal fEPSP and Single Astrocytic Whole-cell Response
12:07
Dual Whole-cell Recordings of Astrocyte and Neuron
13:53
Representative Results
15:45
Conclusion
Summary
Automatic Translation
English (Original)
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français (French)
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italiano (Italian)
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Automatic Translation
编制急性脑切片中分离出海马,以及同步的星形胶质细胞和神经元的电生理记录<em>地层radiatum</em刺激Schaffer侧支时。隔离的星形胶质细胞的钾和谷氨酸转运体电流的药理证明。
Tags
Dual Electrophysiological Recordings
Synaptically-evoked Astroglial Responses
Neuronal Responses
Acute Hippocampal Slices
Tripartite Synapses
Astrocytes
Neuronal Activity Modulation
Ion Channels
Neurotransmitter Receptors
Gliotransmitters
Glutamate Uptake
Potassium Spatial Buffering
GABA Clearance
Synaptic Activity Monitoring
Alterations In Glutamate Levels
Alterations In GABA Levels
Alterations In Extracellular Potassium Levels
Astroglial Uptake Activity
Buffering Capacity
Physiopathological Situations
Knockout Mice
Hippocampal Slice Preparation
Stratum Radiatum Astrocytes Identification
Simultaneous Neuronal And Astroglial Recordings
Synaptically-evoked Astroglial Currents Isolation
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