/
/
Dois registros eletrofisiológicos de sinapticamente evocadas Respostas astroglial e neuronal em fatias hipocampais agudas
This content is Free Access.
26,970 Views
•
16:38 min
•
November 26, 2012
DOI:
Summary
Automatically generated
A preparação de fatias cerebrais agudas de isolado hipocampo, assim como as gravações simultâneas electrofisiológicas de astrócitos e neurónios em<em> Stratum radiatum</em> Durante a estimulação de schaffer colaterais é descrito. O isolamento farmacológica de potássio astroglial e as correntes do transportador de glutamato é demonstrada.
Explore More Videos
Dual Electrophysiological RecordingsSynaptically-evoked Astroglial ResponsesNeuronal ResponsesAcute Hippocampal SlicesTripartite SynapsesAstrocytesNeuronal Activity ModulationIon ChannelsNeurotransmitter ReceptorsGliotransmittersGlutamate UptakePotassium Spatial BufferingGABA ClearanceSynaptic Activity MonitoringAlterations In Glutamate LevelsAlterations In GABA LevelsAlterations In Extracellular Potassium LevelsAstroglial Uptake ActivityBuffering CapacityPhysiopathological SituationsKnockout MiceHippocampal Slice PreparationStratum Radiatum Astrocytes IdentificationSimultaneous Neuronal And Astroglial RecordingsSynaptically-evoked Astroglial Currents Isolation
Read Article
Cite this Article
Pannasch, U., Sibille, J., Rouach, N. Dual Electrophysiological Recordings of Synaptically-evoked Astroglial and Neuronal Responses in Acute Hippocampal Slices. J. Vis. Exp. (69), e4418, doi:10.3791/4418 (2012).
Copy