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Chapters
- 00:05Title
- 02:00Helper-dependent Adenoviral Vector Production and Amplification
- 05:02Large Scale HDAd Production
- 07:13Vector Purification
- 11:19Treatment of Diabetic Mice by HDAd-Ngn3 and -Btc
- 13:08Evidence for Neo-islet Formation in Liver: Representative Results
- 16:14Conclusion
Vi beskriver nedsatt neo-holme formasjonen i STZ (streptozotocin)-indusert diabetiske mus ved genoverføring av Neurogenin3 (Ngn3) og Betacellulin (BTC) med helper-avhengig adenovirusvektor (HDAd) og reversering av hyperglykemi. Vår metode tar fordeler av helper-avhengige adenovirale vektorer med sin svært effektiv in vivo transduksjon og langvarig genuttrykk.
Tags
Neo-Islet FormationLiverDiabetic MiceHelper-dependent Adenoviral Vector-Mediated Gene TransferType 1 DiabetesT Cell-mediated Autoimmune DestructionInsulin-producing CellsPancreasInsulin ReplacementIslet TransplantationDonor AvailabilityLong-term ImmunosuppressionInduced Islet NeogenesisGene TransferNeuogenin3 (Ngn3)Islet Lineage-defining Specific Transcription FactorBetacellulin (Btc)Islet Growth FactorAdenoviral Vectors (Ads)Gene Transfer VectorEarly Generation AdsSafety ProfileTransgene ExpressionChronic ToxicityViral Coding SequencesAd Cis ElementsVector Replication And PackagingProtocolHDAd-Ngn3HDAd-BtcSTZ-induced Diabetic Mice‘neo Islets’LiverHyperglycemia
