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ヘルパー依存型アデノウイルスベクターによる遺伝子導入による糖尿病マウスの肝臓におけるネオ·膵島形成
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Medicine
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JoVE Journal Medicine
Neo-Islet Formation in Liver of Diabetic Mice by Helper-dependent Adenoviral Vector-Mediated Gene Transfer
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ヘルパー依存型アデノウイルスベクターによる遺伝子導入による糖尿病マウスの肝臓におけるネオ·膵島形成

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11,574 Views

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16:59 min

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October 10, 2012

DOI:

10.3791/4321-v

DOI:
10.3791/4321-v

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16:59 min
October 10, 2012

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1 Views
, ,
1Department of Medicine,Baylor College of Medicine , 2Division of Diabetes, Endocrinology & Metabolism, Diabetes & Endocrinology Research Center,Baylor College of Medicine , 3Department of Molecular & Cellular Biology,Baylor College of Medicine

Summary

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我々は、ヘルパー依存性アデノウイルスベクター（HDAd）と高血糖の反転を用いた遺伝子Neurogenin3の転送（Ngn3）とベータセルリン（BTC）によりSTZにおける肝ネオ膵島形成（ストレプトゾトシン）誘発糖尿病マウスを記述します。我々の方法は、生体内伝達および長続きが遺伝子発現におけるそれらの高効率を持つヘルパー依存型アデノウイルスベクターの機能を活用できます。

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Li, R., Oka, K., Yechoor, V. Neo-Islet Formation in Liver of Diabetic Mice by Helper-dependent Adenoviral Vector-Mediated Gene Transfer. J. Vis. Exp. (68), e4321, doi:10.3791/4321 (2012).

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