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新洲形成的助手依赖型腺病毒载体介导的基因转移对糖尿病小鼠肝
JoVE Journal
Medicine
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JoVE Journal Medicine
Neo-Islet Formation in Liver of Diabetic Mice by Helper-dependent Adenoviral Vector-Mediated Gene Transfer
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新洲形成的助手依赖型腺病毒载体介导的基因转移对糖尿病小鼠肝

DOI:
10.3791/4321-v

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16:59 min

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October 10, 2012

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, ,
1Department of Medicine,Baylor College of Medicine , 2Division of Diabetes, Endocrinology & Metabolism, Diabetes & Endocrinology Research Center,Baylor College of Medicine , 3Department of Molecular & Cellular Biology,Baylor College of Medicine

Chapters

  • 00:05Title
  • 02:00Helper-dependent Adenoviral Vector Production and Amplification
  • 05:02Large Scale HDAd Production
  • 07:13Vector Purification
  • 11:19Treatment of Diabetic Mice by HDAd-Ngn3 and -Btc
  • 13:08Evidence for Neo-islet Formation in Liver: Representative Results
  • 16:14Conclusion

Summary

Automatic Translation

Automatic Translation

我们描述了肝形成新胰岛STZ（链脲佐菌素）诱导的糖尿病小鼠的转基因Neurogenin3（Ngn3）和β细胞素（BTC）的使用依赖辅助腺病毒载体（HDAd）和反转高血糖。我们的方法的优点帮手依赖型腺病毒载体，其高度有效的体内转导和持久的基因表达。

Tags

Neo-Islet FormationLiverDiabetic MiceHelper-dependent Adenoviral Vector-Mediated Gene TransferType 1 DiabetesT Cell-mediated Autoimmune DestructionInsulin-producing CellsPancreasInsulin ReplacementIslet TransplantationDonor AvailabilityLong-term ImmunosuppressionInduced Islet NeogenesisGene TransferNeuogenin3 (Ngn3)Islet Lineage-defining Specific Transcription FactorBetacellulin (Btc)Islet Growth FactorAdenoviral Vectors (Ads)Gene Transfer VectorEarly Generation AdsSafety ProfileTransgene ExpressionChronic ToxicityViral Coding SequencesAd Cis ElementsVector Replication And PackagingProtocolHDAd-Ngn3HDAd-BtcSTZ-induced Diabetic Mice‘neo Islets’LiverHyperglycemia

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