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Chapters
- 00:05Title
- 02:00Helper-dependent Adenoviral Vector Production and Amplification
- 05:02Large Scale HDAd Production
- 07:13Vector Purification
- 11:19Treatment of Diabetic Mice by HDAd-Ngn3 and -Btc
- 13:08Evidence for Neo-islet Formation in Liver: Representative Results
- 16:14Conclusion
Biz hepatik neo-adacık STZ formasyonu (streptozotosin)-indüklenen helper bağımlı adenoviral vektör (HDAd) ve hiperglisemi ters kullanılarak gen Neurogenin3 transferi (Ngn3) ve Betacellulin (BTC) ile diyabet oluşturulan farelerde. Tarif Bizim metodumuz vivo iletimi ve uzun ömürlü gen ekspresyonu kendi yüksek verimli olan helper-bağımlı adenoviral vektörlerin avantajları alır.
Tags
Neo-Islet FormationLiverDiabetic MiceHelper-dependent Adenoviral Vector-Mediated Gene TransferType 1 DiabetesT Cell-mediated Autoimmune DestructionInsulin-producing CellsPancreasInsulin ReplacementIslet TransplantationDonor AvailabilityLong-term ImmunosuppressionInduced Islet NeogenesisGene TransferNeuogenin3 (Ngn3)Islet Lineage-defining Specific Transcription FactorBetacellulin (Btc)Islet Growth FactorAdenoviral Vectors (Ads)Gene Transfer VectorEarly Generation AdsSafety ProfileTransgene ExpressionChronic ToxicityViral Coding SequencesAd Cis ElementsVector Replication And PackagingProtocolHDAd-Ngn3HDAd-BtcSTZ-induced Diabetic Mice‘neo Islets’LiverHyperglycemia
