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October 10, 2012
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We beschrijven de lever neo-eilandje formatie in STZ (streptozotocine)-geïnduceerde diabetische muizen door genoverdracht van Neurogenin3 (Ngn3) en betacelluline (BTC) met behulp van helper-afhankelijke adenovirale vector (HDAd) en de omkering van hyperglycemie. Onze methode wordt voordelen van helper-afhankelijke adenovirale vectoren met hun zeer efficiënt in vivo transductie en langdurige genexpressie.
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Neo-Islet FormationLiverDiabetic MiceHelper-dependent Adenoviral Vector-Mediated Gene TransferType 1 DiabetesT Cell-mediated Autoimmune DestructionInsulin-producing CellsPancreasInsulin ReplacementIslet TransplantationDonor AvailabilityLong-term ImmunosuppressionInduced Islet NeogenesisGene TransferNeuogenin3 (Ngn3)Islet Lineage-defining Specific Transcription FactorBetacellulin (Btc)Islet Growth FactorAdenoviral Vectors (Ads)Gene Transfer VectorEarly Generation AdsSafety ProfileTransgene ExpressionChronic ToxicityViral Coding SequencesAd Cis ElementsVector Replication And PackagingProtocolHDAd-Ngn3HDAd-BtcSTZ-induced Diabetic Mice‘neo Islets’LiverHyperglycemia
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Li, R., Oka, K., Yechoor, V. Neo-Islet Formation in Liver of Diabetic Mice by Helper-dependent Adenoviral Vector-Mediated Gene Transfer. J. Vis. Exp. (68), e4321, doi:10.3791/4321 (2012).
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