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El examen de selección tímica positivo y negativo por citometría de flujo
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October 08, 2012
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Se presenta una citometría de flujo basado en el método para examinar el desarrollo de las células T<em> In vivo</em> Utilizando ratones genéticamente manipulados en un fondo de tipo salvaje o receptor de células T transgénicos.
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Thymic Positive SelectionThymic Negative SelectionFlow CytometryT Cell Receptor (TCR)Antigen SpecificitySelf-antigensT Cell RepertoireAutoimmune DisordersImmunodeficiency DisordersDouble Negative (DN) ThymocytesDouble Positive (DP) StageCD4 Co-receptorCD8 Co-receptorSelf-peptide-MHC (pMHC)High Affinity InteractionsLow Affinity InteractionsPositive SelectionSingle Positive (SP) T CellsForeign AntigensPolyclonal TCR Repertoire
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Hu, Q., Nicol, S. A., Suen, A. Y., Baldwin, T. A. Examination of Thymic Positive and Negative Selection by Flow Cytometry. J. Vis. Exp. (68), e4269, doi:10.3791/4269 (2012).
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