/
/
Exame de seleção positiva e negativa Tímico por citometria de fluxo
A subscription to JoVE is required to view this content. Sign in or start your free trial.
21,494 Views
•
14:29 min
•
October 08, 2012
DOI:
Summary
Automatically generated
Apresenta-se um método de citometria de fluxo com base para examinar o desenvolvimento das células T<em> In vivo</em> Usando camundongos geneticamente manipulados em um tipo selvagem ou de fundo do receptor de células T transgênico.
Explore More Videos
Thymic Positive SelectionThymic Negative SelectionFlow CytometryT Cell Receptor (TCR)Antigen SpecificitySelf-antigensT Cell RepertoireAutoimmune DisordersImmunodeficiency DisordersDouble Negative (DN) ThymocytesDouble Positive (DP) StageCD4 Co-receptorCD8 Co-receptorSelf-peptide-MHC (pMHC)High Affinity InteractionsLow Affinity InteractionsPositive SelectionSingle Positive (SP) T CellsForeign AntigensPolyclonal TCR Repertoire
Read Article
Cite this Article
Hu, Q., Nicol, S. A., Suen, A. Y., Baldwin, T. A. Examination of Thymic Positive and Negative Selection by Flow Cytometry. J. Vis. Exp. (68), e4269, doi:10.3791/4269 (2012).
Copy