このコンテンツを視聴するには、JoVE 購読が必要です。 サインイン又は無料トライアルを申し込む。
Examination of Thymic Positive and Negative Selection by Flow Cytometry
21,494 Views
•
14:29 min
•
October 08, 2012
DOI:
概要
Automatically generated
We present a flow cytometry-based method to examine T cell development in vivo using genetically manipulated mice on a wildtype or T cell receptor transgenic background.
Explore More Videos
Thymic Positive SelectionThymic Negative SelectionFlow CytometryT Cell Receptor (TCR)Antigen SpecificitySelf-antigensT Cell RepertoireAutoimmune DisordersImmunodeficiency DisordersDouble Negative (DN) ThymocytesDouble Positive (DP) StageCD4 Co-receptorCD8 Co-receptorSelf-peptide-MHC (pMHC)High Affinity InteractionsLow Affinity InteractionsPositive SelectionSingle Positive (SP) T CellsForeign AntigensPolyclonal TCR Repertoire
Read Article
Cite this Article
Hu, Q., Nicol, S. A., Suen, A. Y., Baldwin, T. A. Examination of Thymic Positive and Negative Selection by Flow Cytometry. J. Vis. Exp. (68), e4269, doi:10.3791/4269 (2012).
Copy