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Examination of Thymic Positive and Negative Selection by Flow Cytometry
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Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Examination of Thymic Positive and Negative Selection by Flow Cytometry

Examination of Thymic Positive and Negative Selection by Flow Cytometry

DOI:
10.3791/4269-v

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14:29 min

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October 08, 2012

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, , ,
1Department of Medical Microbiology and Immunology,University of Alberta

Capitoli

  • 00:05Titolo
  • 01:16Dissection
  • 02:51Cell Preparation
  • 04:06Staining Cells for Flow Cytometry
  • 05:22Results: Thymic Positive and Negative Selection by Flow Cytometry
  • 13:37Conclusion

Summary

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We present a flow cytometry-based method to examine T cell development in vivo using genetically manipulated mice on a wildtype or T cell receptor transgenic background.

Tags

Thymic Positive SelectionThymic Negative SelectionFlow CytometryT Cell Receptor (TCR)Antigen SpecificitySelf-antigensT Cell RepertoireAutoimmune DisordersImmunodeficiency DisordersDouble Negative (DN) ThymocytesDouble Positive (DP) StageCD4 Co-receptorCD8 Co-receptorSelf-peptide-MHC (pMHC)High Affinity InteractionsLow Affinity InteractionsPositive SelectionSingle Positive (SP) T CellsForeign AntigensPolyclonal TCR Repertoire

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