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High Throughput ELISA sequencial para a validação dos biomarcadores da doença enxerto-versus-hospedeiro aguda
JoVE Journal
Medicine
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JoVE Journal Medicine
High Throughput Sequential ELISA for Validation of Biomarkers of Acute Graft-Versus-Host Disease

High Throughput ELISA sequencial para a validação dos biomarcadores da doença enxerto-versus-hospedeiro aguda

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15,560 Views

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09:00 min

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October 31, 2012

DOI:

10.3791/4247-v

DOI:
10.3791/4247-v

•

09:00 min
October 31, 2012

•

7 Views
, , , , , ,
1Pediatric Blood and Marrow Transplant Program,University of Michigan

Summary

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Validação com rendimento elevado de candidatos a biomarcadores múltiplos pode ser realizada por meio de ELISA sequencial de modo a minimizar de congelamento / descongelamento e utilização de amostras de plasma preciosos. Aqui, nós demonstrar como executar sequencialmente ELISAs para seis diferentes biomarcadores de plasma validados<sup> 1-3</sup> De enxerto-versus-hospedeiro (GVHD)<sup> 4</sup> Na amostra de plasma mesmo.

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Cite this Article

Fiema, B., Harris, A. C., Gomez, A., Pongtornpipat, P., Lamiman, K., Vander Lugt, M. T., Paczesny, S. High Throughput Sequential ELISA for Validation of Biomarkers of Acute Graft-Versus-Host Disease. J. Vis. Exp. (68), e4247, doi:10.3791/4247 (2012).

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