Waiting
Login-Verarbeitung ...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Medicine / High Throughput Sequential ELISA for Validation…
JoVE Journal
Medicine

Dieser Inhalt ist Open Access.

JoVE Journal Medicine
High Throughput Sequential ELISA for Validation of Biomarkers of Acute Graft-Versus-Host Disease
 

High Throughput Sequential ELISA for Validation of Biomarkers of Acute Graft-Versus-Host Disease

Article DOI: 10.3791/4247-v • 09:00 min • October 31st, 2012
October 31st, 2012 •
*1, *1, 1, 1, 1, 1, 1
1Pediatric Blood and Marrow Transplant Program, University of Michigan
* These authors contributed equally

Kapitel

Summary
Automatic Translation

High throughput validation of multiple candidate biomarkers can be performed by sequential ELISA in order to minimize freeze/thaw cycles and use of precious plasma samples. Here, we demonstrate how to sequentially perform ELISAs for six different validated plasma biomarkers1-3 of graft-versus-host disease (GVHD)4 on the same plasma sample.

Tags

High Throughput Sequential ELISA Validation Of Biomarkers Acute Graft-Versus-Host Disease Unbiased Discovery Proteomics Strategies Novel Biomarkers Diagnostic Testing Prognostic Testing Therapeutic Interventions Candidate Proteins Validate Candidate Biomarkers Patient Plasma Samples Acute Graft-versus-host Disease Allogeneic Hematopoietic Stem Cell Transplantation ELISAs GVHD Proteins IL-2Rα5 TNFR16 HGF7 IL-88 Elafin2 REG3α3 PAP1 Sequential ELISA Performance Freeze-thaw Cycles Thawed Plasma Time Plasma Usage Plasma Biomarker Concentrations
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter