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High Throughput Sequential ELISA for Validation of Biomarkers of Acute Graft-Versus-Host Disease
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October 31, 2012
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High throughput validation of multiple candidate biomarkers can be performed by sequential ELISA in order to minimize freeze/thaw cycles and use of precious plasma samples. Here, we demonstrate how to sequentially perform ELISAs for six different validated plasma biomarkers1-3 of graft-versus-host disease (GVHD)4 on the same plasma sample.
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Fiema, B., Harris, A. C., Gomez, A., Pongtornpipat, P., Lamiman, K., Vander Lugt, M. T., Paczesny, S. High Throughput Sequential ELISA for Validation of Biomarkers of Acute Graft-Versus-Host Disease. J. Vis. Exp. (68), e4247, doi:10.3791/4247 (2012).
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