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October 31, 2012
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Convalida elevato throughput di biomarcatori candidati multipli può essere eseguita da ELISA sequenziale per minimizzare gelo / disgelo e l'uso di campioni di plasma preziosi. Qui, dimostriamo come eseguire in sequenza ELISA per sei diversi biomarcatori plasmatici convalidati<sup> 1-3</sup> Di graft-versus-host disease (GVHD)<sup> 4</sup> Il campione di plasma stesso.
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Fiema, B., Harris, A. C., Gomez, A., Pongtornpipat, P., Lamiman, K., Vander Lugt, M. T., Paczesny, S. High Throughput Sequential ELISA for Validation of Biomarkers of Acute Graft-Versus-Host Disease. J. Vis. Exp. (68), e4247, doi:10.3791/4247 (2012).
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