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区分功能基因表达的作用，通过骨髓移植免疫和非免疫细胞在小鼠结肠炎
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Immunology and Infection
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JoVE Journal Immunology and Infection
Differentiating Functional Roles of Gene Expression from Immune and Non-immune Cells in Mouse Colitis by Bone Marrow Transplantation
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区分功能基因表达的作用，通过骨髓移植免疫和非免疫细胞在小鼠结肠炎

DOI:
10.3791/4208-v

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14:43 min

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October 01, 2012

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, , , ,
1Center for Inflammatory Bowel Diseases, Division of Digestive Diseases, David Geffen School of Medicine,The University of California Los Angeles, Los Angeles

Chapters

  • 00:05Title
  • 01:26Experimental Setup
  • 02:39Donor Bone Marrow Extraction
  • 05:49Infusion to Recipient Mice
  • 07:19Induction of Colitis and Evaluation of Colitis
  • 08:55Quality Inspection of Bone Marrow Transplantation by Flow Cytometry
  • 11:59Transplantation Results
  • 14:21Conclusion

Summary

Automatic Translation

Automatic Translation

骨髓移植提供了一种方法来改变的骨髓衍生细胞的基因型。如果感兴趣的基因的表达在两个骨髓来源的细胞和非骨髓来源的细胞，骨髓移植可以改变到一个不同的基因型的骨髓来源的细胞，不改变非骨髓来源的细胞的基因型。

Tags

Gene ExpressionImmune CellsNon-immune CellsMouse ColitisBone Marrow TransplantationGene-of-interestKnockout MiceGenotypeIrradiationDonor Bone MarrowRecipient MiceWild-type MiceCD Molecules (CD45.1 And CD45.2)

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