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An In Vivo Mouse Model to Measure Naïve CD4 T Cell Activation, Proliferation and Th1 Differentiation Induced by Bone Marrow-derived Dendritic Cells
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Chapters
- 00:04Title
- 00:46Mouse Bone Marrow (BM) and Secondary Lymphoid Tissue Cell Isolation
- 03:40In Vivo Activation, Proliferation, and Th1 Cell Differentiation Assay
- 06:48Results: Representative BM-derived Dentritic Cell-induced Naïve CD4+ T Cell Activation, Proliferation, and Th1 Differentiation
- 07:39Conclusion
Here, we present a protocol for the in vivo determination of naïve CD4 T cell (T cell) activation, proliferation, and Th1 differentiation induced by GM-CSF bone marrow (BM)-derived dendritic cells (DCs). In addition, this protocol describes BM and T-cell isolation, DC generation, and DC and T-cell adoptive transfer.
