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Valutare mutazione somatica nel Ramos cellule B dopo iperespressione o Knockdown di specifici geni
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08:12 min
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November 01, 2011
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Summary
Automatically generated
Ci descrivono come eseguire infezioni retrovirali o lentivirali di sovraespressione o shRNA contenenti costruisce nell'essere umano a cellule B Ramos line e come misurare mutazione somatica in queste cellule.
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Somatic HypermutationRamos B CellsOverexpressionKnockdownSpecific GenesV(D)J RecombinationAffinity AntibodiesImmunoglobulin GeneSomatic Hypermutation (SHM)Class Switch Recombination (CSR)Activation-induced Cytidine Deaminase (AID)Cytosine ResiduesUracilsRepair PathwaysMolecular DetailsMice ModelsGenetic KnockoutsRepair FactorsDouble-strand Break Repair ProteinsB-cell Development
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Cite this Article
Upton, D. C., Unniraman, S. Assessing Somatic Hypermutation in Ramos B Cells after Overexpression or Knockdown of Specific Genes. J. Vis. Exp. (57), e3573, doi:10.3791/3573 (2011).
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