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Assessing Somatic Hypermutation in Ramos B Cells after Overexpression or Knockdown of Specific Genes
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November 01, 2011
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We describe how to perform retroviral or lentiviral infections of overexpression or shRNA-containing constructs in the human Ramos B-cell line and how to measure somatic hypermutation in these cells.
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Somatic HypermutationRamos B CellsOverexpressionKnockdownSpecific GenesV(D)J RecombinationAffinity AntibodiesImmunoglobulin GeneSomatic Hypermutation (SHM)Class Switch Recombination (CSR)Activation-induced Cytidine Deaminase (AID)Cytosine ResiduesUracilsRepair PathwaysMolecular DetailsMice ModelsGenetic KnockoutsRepair FactorsDouble-strand Break Repair ProteinsB-cell Development
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Upton, D. C., Unniraman, S. Assessing Somatic Hypermutation in Ramos B Cells after Overexpression or Knockdown of Specific Genes. J. Vis. Exp. (57), e3573, doi:10.3791/3573 (2011).
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