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Assessing Somatic Hypermutation in Ramos B Cells after Overexpression or Knockdown of Specific Genes
JoVE Revista
Inmunología y contagio
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JoVE Revista Inmunología y contagio
Assessing Somatic Hypermutation in Ramos B Cells after Overexpression or Knockdown of Specific Genes

Assessing Somatic Hypermutation in Ramos B Cells after Overexpression or Knockdown of Specific Genes

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08:12 min

November 01, 2011

DOI:

08:12 min
November 01, 2011

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Summary

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We describe how to perform retroviral or lentiviral infections of overexpression or shRNA-containing constructs in the human Ramos B-cell line and how to measure somatic hypermutation in these cells.

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