/
/
Assessing Somatic Hypermutation in Ramos B Cells after Overexpression or Knockdown of Specific Genes
È necessario avere un abbonamento a JoVE per visualizzare questo. Accedi o inizia la tua prova gratuita.
Assessing Somatic Hypermutation in Ramos B Cells after Overexpression or Knockdown of Specific Genes
18,022 Views
•
08:12 min
•
November 01, 2011
DOI:
Summary
Automatically generated
We describe how to perform retroviral or lentiviral infections of overexpression or shRNA-containing constructs in the human Ramos B-cell line and how to measure somatic hypermutation in these cells.
Explore More Videos
Somatic HypermutationRamos B CellsOverexpressionKnockdownSpecific GenesV(D)J RecombinationAffinity AntibodiesImmunoglobulin GeneSomatic Hypermutation (SHM)Class Switch Recombination (CSR)Activation-induced Cytidine Deaminase (AID)Cytosine ResiduesUracilsRepair PathwaysMolecular DetailsMice ModelsGenetic KnockoutsRepair FactorsDouble-strand Break Repair ProteinsB-cell Development
Read Article
Cite this Article
Upton, D. C., Unniraman, S. Assessing Somatic Hypermutation in Ramos B Cells after Overexpression or Knockdown of Specific Genes. J. Vis. Exp. (57), e3573, doi:10.3791/3573 (2011).
Copy