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Aislamiento de células dendríticas del pulmón de ratón
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Isolation of Mouse Lung Dendritic Cells
DOI:

09:04 min

November 22, 2011

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Chapters

  • 00:05Title
  • 01:20Lung Perfusion and Single Cell Suspension
  • 03:48Alternative Protocol for Obtaining a Single Cell Suspension
  • 04:26Magnetic Isolation and CD11c+ Cell Enrichment
  • 05:45Conventional Dendritic Cell Isolation
  • 06:46Combining Magnetic Bead Isolation and Flow Cytometric Cell Sorting Results in an Highly Purified Lung Conventional Dendritic Cell Population
  • 08:16Conclusion

Summary

Automatic Translation

Una preparación altamente purificada de células dendríticas del pulmón del ratón se describe. Especial énfasis se da al aislamiento de subgrupo de células dendríticas convencionales.

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