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Met behulp van reverse genetics aan de NSS-gen van de Rift Valley Fever virus MP-12 Strain om Vaccine veiligheid en effectiviteit te verbeteren manipuleren
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09:13 min
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November 01, 2011
DOI:
Summary
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De reverse genetics systeem voor de Rift Valley koorts virus MP-12 vaccinstam is een handig hulpmiddel voor het creëren van extra MP-12 mutanten met verhoogde verzwakking en immunogeniciteit. We beschrijven het protocol te genereren en karakteriseren NSS mutante stammen.
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Reverse GeneticsNSs GeneRift Valley Fever VirusMP-12 StrainVaccine SafetyVaccine EfficacyHemorrhagic FeverNeurological DisordersBlindnessAbortionFetal MalformationHHS/USDA Overlap Select AgentRisk Group 3 PathogenPhlebovirusBunyaviridae FamilyVirulentRVFV MP-12 Vaccine StrainWild-type RVFV StrainsZH548ZH501M-segmentL-segmentS-segment RNA3Functional Virulence Factor NSsRMP12-C13typeIn-frame Deletion Of NSs ORFHost Transcription Shut-offInterferon (IFN)-beta MRNA78Double-stranded RNA-dependent Protein Kinase (PKR)Post-translational Level Degradation Of PKR
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Kalveram, B., Lihoradova, O., Indran, S. V., Ikegami, T. Using Reverse Genetics to Manipulate the NSs Gene of the Rift Valley Fever Virus MP-12 Strain to Improve Vaccine Safety and Efficacy. J. Vis. Exp. (57), e3400, doi:10.3791/3400 (2011).
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