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Using Reverse Genetics to Manipulate the NSs Gene of the Rift Valley Fever Virus MP-12 Strain to Improve Vaccine Safety and Efficacy
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November 01, 2011
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Summary
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The reverse genetics system for the Rift Valley fever virus MP-12 vaccine strain is a useful tool for creating additional MP-12 mutants with increased attenuation and immunogenicity. We describe the protocol to generate and characterize NSs mutant strains.
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Reverse GeneticsNSs GeneRift Valley Fever VirusMP-12 StrainVaccine SafetyVaccine EfficacyHemorrhagic FeverNeurological DisordersBlindnessAbortionFetal MalformationHHS/USDA Overlap Select AgentRisk Group 3 PathogenPhlebovirusBunyaviridae FamilyVirulentRVFV MP-12 Vaccine StrainWild-type RVFV StrainsZH548ZH501M-segmentL-segmentS-segment RNA3Functional Virulence Factor NSsRMP12-C13typeIn-frame Deletion Of NSs ORFHost Transcription Shut-offInterferon (IFN)-beta MRNA78Double-stranded RNA-dependent Protein Kinase (PKR)Post-translational Level Degradation Of PKR
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Kalveram, B., Lihoradova, O., Indran, S. V., Ikegami, T. Using Reverse Genetics to Manipulate the NSs Gene of the Rift Valley Fever Virus MP-12 Strain to Improve Vaccine Safety and Efficacy. J. Vis. Exp. (57), e3400, doi:10.3791/3400 (2011).
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