Rivista
/
Immunologia e infezioni
/
Using Reverse Genetics to Manipulate the NSs Gene of the Rift Valley Fever Virus MP-12 Strain to Improve Vaccine Safety and Efficacy
JoVE Journal
Immunologia e infezioni
È necessario avere un abbonamento a JoVE per visualizzare questo.  Accedi o inizia la tua prova gratuita.
JoVE Journal Immunologia e infezioni
Using Reverse Genetics to Manipulate the NSs Gene of the Rift Valley Fever Virus MP-12 Strain to Improve Vaccine Safety and Efficacy

Using Reverse Genetics to Manipulate the NSs Gene of the Rift Valley Fever Virus MP-12 Strain to Improve Vaccine Safety and Efficacy

DOI:
10.3791/3400-v

•

09:13 min

•

November 01, 2011

•

, , ,
1Department of Pathology,University of Texas Medical Branch

Capitoli

  • 00:05Titolo
  • 01:57Preparation of Recombinant MP-12 Encoding NSs Mutations
  • 03:38Amplification of P0 Virus
  • 04:16Titration of Recombinant MP-12 by Plaque Assay
  • 06:01Screening of NSs Mutants Lacking the Type-I IFN Suppression Function
  • 07:03Reverse Genetics Approach to the C13type MP-12 Virus
  • 08:22Conclusion

Summary

Traduzione automatica

Traduzione automatica

The reverse genetics system for the Rift Valley fever virus MP-12 vaccine strain is a useful tool for creating additional MP-12 mutants with increased attenuation and immunogenicity. We describe the protocol to generate and characterize NSs mutant strains.

Tags

Reverse GeneticsNSs GeneRift Valley Fever VirusMP-12 StrainVaccine SafetyVaccine EfficacyHemorrhagic FeverNeurological DisordersBlindnessAbortionFetal MalformationHHS/USDA Overlap Select AgentRisk Group 3 PathogenPhlebovirusBunyaviridae FamilyVirulentRVFV MP-12 Vaccine StrainWild-type RVFV StrainsZH548ZH501M-segmentL-segmentS-segment RNA3Functional Virulence Factor NSsRMP12-C13typeIn-frame Deletion Of NSs ORFHost Transcription Shut-offInterferon (IFN)-beta MRNA78Double-stranded RNA-dependent Protein Kinase (PKR)Post-translational Level Degradation Of PKR

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

Reverse Genetics Mediated Recovery of Infectious Murine Norovirus

Using Click Chemistry to Measure the Effect of Viral Infection on Host-Cell RNA Synthesis

Cell-based Flow Cytometry Assay to Measure Cytotoxic Activity

In Vitro Analysis of Myd88-mediated Cellular Immune Response to West Nile Virus Mutant Strain Infection

Evaluation of the Efficacy And Toxicity of RNAs Targeting HIV-1 Production for Use in Gene or Drug Therapy

Generation of Escape Variants of Neutralizing Influenza Virus Monoclonal Antibodies

Overexpression and Purification of Human Cis-prenyltransferase in Escherichia coli

Using Phylogenetic Analysis to Investigate Eukaryotic Gene Origin

Assembly and Purification of Prototype Foamy Virus Intasomes

Evaluation of Host-Pathogen Responses and Vaccine Efficacy in Mice

Read Article