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Using Reverse Genetics to Manipulate the NSs Gene of the Rift Valley Fever Virus MP-12 Strain to Improve Vaccine Safety and Efficacy
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Immunology and Infection
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JoVE Journal Immunology and Infection
Using Reverse Genetics to Manipulate the NSs Gene of the Rift Valley Fever Virus MP-12 Strain to Improve Vaccine Safety and Efficacy

Using Reverse Genetics to Manipulate the NSs Gene of the Rift Valley Fever Virus MP-12 Strain to Improve Vaccine Safety and Efficacy

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17,201 Views

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09:13 min

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November 01, 2011

DOI:

10.3791/3400-v

DOI:
10.3791/3400-v

•

09:13 min
November 01, 2011

•

3 Views
, , ,
1Department of Pathology,University of Texas Medical Branch

Summary

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The reverse genetics system for the Rift Valley fever virus MP-12 vaccine strain is a useful tool for creating additional MP-12 mutants with increased attenuation and immunogenicity. We describe the protocol to generate and characterize NSs mutant strains.

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Kalveram, B., Lihoradova, O., Indran, S. V., Ikegami, T. Using Reverse Genetics to Manipulate the NSs Gene of the Rift Valley Fever Virus MP-12 Strain to Improve Vaccine Safety and Efficacy. J. Vis. Exp. (57), e3400, doi:10.3791/3400 (2011).

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