Establishment of Epstein-Barr Virus Growth-transformed Lymphoblastoid Cell Lines

Joyce Hui-Yuen; Shane McAllister; Siva Koganti; Erik Hill; Sumita Bhaduri-McIntosh

doi:10.3791/3321

Journal

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Immunology and Infection

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Estabelecimento de Epstein-Barr Virus Crescimento transformada linhagens de células linfoblastóides

JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection

Establishment of Epstein-Barr Virus Growth-transformed Lymphoblastoid Cell Lines

Estabelecimento de Epstein-Barr Virus Crescimento transformada linhagens de células linfoblastóides

39,416 Views

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06:38 min

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November 08, 2011

DOI:

10.3791/3321-v

DOI:

10.3791/3321-v

•

06:38 min

November 08, 2011

•

17 Views

Joyce Hui-Yuen^1,2, Shane McAllister^1,2, Siva Koganti², Erik Hill², Sumita Bhaduri-McIntosh^1,2,3,4

¹Stony Brook Children’s Hospital,State University of New York at Stony Brook, ²Department of Pediatrics,State University of New York at Stony Brook, ³Department of Molecular Genetics,State University of New York at Stony Brook, ⁴Department of Microbiology,State University of New York at Stony Brook

Summary

Nós descrevemos um método para gerar linhas B transformada célula usando vírus Epstein-Barr. Nós também ilustrar um ensaio de romance que pode identificar as células B destinados a sofrer transformações logo aos três dias após a infecção.

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Epstein-Barr Virus Growth-transformed Lymphoblastoid Cell Lines EBV Infection B Cell Proliferation Immortalization EBV Latency Virus-driven B Cell Proliferation Tumorigenesis Immunologic Assays Human Monoclonal Antibodies Mitogens Phytohemagglutinin Lipopolysaccharide Pokeweed Mitogen Cyclosporin A T Cell-mediated Killing High Titer EBV Immunosuppressive Agent Peripheral Blood Mononuclear Cells

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Estabelecimento de Epstein-Barr Virus Crescimento transformada linhagens de células linfoblastóides

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