Dergi
/
İmmünoloji ve Enfeksiyon
/
Establishment of Epstein-Barr Virus Growth-transformed Lymphoblastoid Cell Lines
JoVE Journal
İmmünoloji ve Enfeksiyon
This content is Free Access.
JoVE Journal İmmünoloji ve Enfeksiyon
Establishment of Epstein-Barr Virus Growth-transformed Lymphoblastoid Cell Lines

Establishment of Epstein-Barr Virus Growth-transformed Lymphoblastoid Cell Lines

39,399 Views

•

06:38 min

•

November 08, 2011

DOI:

10.3791/3321-v

DOI:
10.3791/3321-v

•

06:38 min
November 08, 2011

•

17 Views
, , , ,
1Stony Brook Children’s Hospital,State University of New York at Stony Brook, 2Pediatri Anabilim Dalı,State University of New York at Stony Brook, 3Department of Molecular Genetics,State University of New York at Stony Brook, 4Department of Microbiology,State University of New York at Stony Brook

Özet

Automatically generated

We describe a method for generating transformed B cell lines using Epstein-Barr virus. We also illustrate a novel assay that can identify B cells destined to undergo transformation as early as three days after infection.

Explore More Videos

Epstein-Barr VirusGrowth-transformed Lymphoblastoid Cell LinesEBV InfectionB Cell ProliferationImmortalizationEBV LatencyVirus-driven B Cell ProliferationTumorigenesisImmunologic AssaysHuman Monoclonal AntibodiesMitogensPhytohemagglutininLipopolysaccharidePokeweed MitogenCyclosporin AT Cell-mediated KillingHigh Titer EBVImmunosuppressive AgentPeripheral Blood Mononuclear Cells

Read Article

Cite this Article

Hui-Yuen, J., McAllister, S., Koganti, S., Hill, E., Bhaduri-McIntosh, S. Establishment of Epstein-Barr Virus Growth-transformed Lymphoblastoid Cell Lines. J. Vis. Exp. (57), e3321, doi:10.3791/3321 (2011).

Download .ris file

Copy

Share Video

.

Copy