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Immunology and Infection
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Création d'Epstein-Barr Virus croissance transformées lignées lymphoblastoïdes
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Establishment of Epstein-Barr Virus Growth-transformed Lymphoblastoid Cell Lines
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Création d'Epstein-Barr Virus croissance transformées lignées lymphoblastoïdes

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39,411 Views

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06:38 min

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November 08, 2011

DOI:

10.3791/3321-v

DOI:
10.3791/3321-v

•

06:38 min
November 08, 2011

•

17 Views
, , , ,
1Stony Brook Children’s Hospital,State University of New York at Stony Brook, 2Department of Pediatrics,State University of New York at Stony Brook, 3Department of Molecular Genetics,State University of New York at Stony Brook, 4Department of Microbiology,State University of New York at Stony Brook

Summary

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Nous décrivons une méthode pour générer des lignes cellulaires transformées B à l'aide d'Epstein-Barr virus. Nous avons également illustrer un nouveau test qui permet d'identifier les cellules B destiné à subir une transformation aussi tôt que trois jours après l'infection.

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Hui-Yuen, J., McAllister, S., Koganti, S., Hill, E., Bhaduri-McIntosh, S. Establishment of Epstein-Barr Virus Growth-transformed Lymphoblastoid Cell Lines. J. Vis. Exp. (57), e3321, doi:10.3791/3321 (2011).

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