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Cultura e Eletrofisiologia de células em NRCC patch-clamp-Chips
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Neuroscience
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JoVE Journal Neuroscience
Culturing and Electrophysiology of Cells on NRCC Patch-clamp Chips

Cultura e Eletrofisiologia de células em NRCC patch-clamp-Chips

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15,496 Views

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10:36 min

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February 07, 2012

DOI:

10.3791/3288-v

DOI:
10.3791/3288-v

•

10:36 min
February 07, 2012

•

8 Views
, , , , , , ,
1Institute for Microstructural Sciences,National Research Council of Canada, 2Institute for Biological Sciences,National Research Council of Canada, 3Hotchkiss Brain Institute,University of Calgary

Summary

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Nós mostramos como planar de patch-clamp fichas fabricado no National Research Council of Canada são esterilizados, preparado, carregado com médio, revestida com as células, e usado para gravações eletrofisiológicas.

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Cite this Article

Py, C., Martina, M., Monette, R., Comas, T., Denhoff, M. W., Luk, C., Syed, N. I., Mealing, G. Culturing and Electrophysiology of Cells on NRCC Patch-clamp Chips. J. Vis. Exp. (60), e3288, doi:10.3791/3288 (2012).

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