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Culturing and Electrophysiology of Cells on NRCC Patch-clamp Chips
JoVE 杂志
神经科学
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JoVE 杂志 神经科学
Culturing and Electrophysiology of Cells on NRCC Patch-clamp Chips

Culturing and Electrophysiology of Cells on NRCC Patch-clamp Chips

15,491 Views

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10:36 min

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February 07, 2012

DOI:

10.3791/3288-v

DOI:
10.3791/3288-v

•

10:36 min
February 07, 2012

•

8 Views
, , , , , , ,
1Institute for Microstructural Sciences,National Research Council of Canada, 2Institute for Biological Sciences,National Research Council of Canada, 3Hotchkiss Brain Institute,University of Calgary

Summary

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We show how planar patch-clamp chips fabricated at the National Research Council of Canada are sterilized, primed, loaded with medium, plated with cells, and used for electrophysiological recordings.

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Cite this Article

Py, C., Martina, M., Monette, R., Comas, T., Denhoff, M. W., Luk, C., Syed, N. I., Mealing, G. Culturing and Electrophysiology of Cells on NRCC Patch-clamp Chips. J. Vis. Exp. (60), e3288, doi:10.3791/3288 (2012).

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