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Die Züchtung und Elektrophysiologie von Zellen auf NRCC Patch-Clamp-Chips
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Culturing and Electrophysiology of Cells on NRCC Patch-clamp Chips
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Die Züchtung und Elektrophysiologie von Zellen auf NRCC Patch-Clamp-Chips

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15,496 Views

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10:36 min

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February 07, 2012

DOI:

10.3791/3288-v

DOI:
10.3791/3288-v

•

10:36 min
February 07, 2012

•

8 Views
, , , , , , ,
1Institute for Microstructural Sciences,National Research Council of Canada, 2Institute for Biological Sciences,National Research Council of Canada, 3Hotchkiss Brain Institute,University of Calgary

Summary

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Wir zeigen, wie planaren Patch-Clamp-Chips an der National Research Council von Kanada werden sterilisiert, grundiert, mit Medium geladen, mit Zellen ausplattiert, hergestellt und verwendet für elektrophysiologische Ableitungen.

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Cite this Article

Py, C., Martina, M., Monette, R., Comas, T., Denhoff, M. W., Luk, C., Syed, N. I., Mealing, G. Culturing and Electrophysiology of Cells on NRCC Patch-clamp Chips. J. Vis. Exp. (60), e3288, doi:10.3791/3288 (2012).

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