Dergi
/
Nörobilim
/
Culturing and Electrophysiology of Cells on NRCC Patch-clamp Chips
JoVE Journal
Nörobilim
This content is Free Access.
JoVE Journal Nörobilim
Culturing and Electrophysiology of Cells on NRCC Patch-clamp Chips

Culturing and Electrophysiology of Cells on NRCC Patch-clamp Chips

DOI:
10.3791/3288-v

•

10:36 min

•

February 07, 2012

•

, , , , , , ,
1Institute for Microstructural Sciences,National Research Council of Canada, 2Institute for Biological Sciences,National Research Council of Canada, 3Hotchkiss Brain Institute,University of Calgary

Bölümler

  • 00:05Başlık
  • 02:49Sterilization, Priming and Testing of Patch-clamp Chips
  • 04:14Chips Preparation in Cell Biology Lab
  • 07:07Cell Plating of Snail Neurons
  • 08:45Electrophysiological Recordings
  • 09:09Representative Results for the Electrophysiology of Cells on NRCC Patch-clamp Chips
  • 09:22Conclusion

Özet

Otomatik Çeviri

Otomatik Çeviri

We show how planar patch-clamp chips fabricated at the National Research Council of Canada are sterilized, primed, loaded with medium, plated with cells, and used for electrophysiological recordings.

Etiketler

CulturingElektrofizyolojiCellsPatch-clampChipsSensitivityIon ChannelsGold StandardDisease ModelsPharmaceutical ScreensGlass PipetteMembrane PatchElectrodeIon-channel ActivityWhole CellPatch-clamp ChipsSuspended FilmAperture MicrofabricatedAutomated SystemsHigh-throughput ScreeningFluidic DeliverySuctionCell-to-probe SealsWhole Cell ModeSilicon Patch-clamp ChipImpedanceOrifice ShapeAction PotentialsSnail NeuronsMammalian Neurons

Article

Embed

ÇALMA LİSTESİNE EKLE

Usage Statistics

İlgili Videolar

Paired Patch Clamp Recordings from Motor-neuron and Target Skeletal Muscle in Zebrafish

Patch-clamp Capacitance Measurements and Ca2+ Imaging at Single Nerve Terminals in Retinal Slices

Patch Clamp Recordings in Inner Ear Hair Cells Isolated from Zebrafish

Whole Cell Patch Clamp for Investigating the Mechanisms of Infrared Neural Stimulation

Patch Clamp Recordings from Embryonic Zebrafish Mauthner Cells

A Computer-assisted Multi-electrode Patch-clamp System

Whole-cell Patch-clamp Recordings from Morphologically- and Neurochemically-identified Hippocampal Interneurons

Whole-cell Patch-clamp Recordings in Brain Slices

Patch Clamp Recording of Starburst Amacrine Cells in a Flat-mount Preparation of Deafferentated Mouse Retina

Whole-cell Patch-clamp Recordings for Electrophysiological Determination of Ion Selectivity in Channelrhodopsins

Read Article