Isolation of CD133+ Liver Stem Cells for Clonal Expansion

C. Bart Rountree; Wei Ding; Hein Dang; Colleen VanKirk; Gay M. Crooks

doi:10.3791/3183

Journal

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Biology

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Klonal Genişleme CD133 + Karaciğer Kök Hücre İzolasyonu

JoVE Journal
Biology

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JoVE Journal Biology

Isolation of CD133+ Liver Stem Cells for Clonal Expansion

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Klonal Genişleme CD133 + Karaciğer Kök Hücre İzolasyonu

22,056 Views

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12:06 min

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October 10, 2011

DOI:

10.3791/3183-v

DOI:

10.3791/3183-v

•

12:06 min

October 10, 2011

•

4 Views

C. Bart Rountree¹, Wei Ding¹, Hein Dang¹, Colleen VanKirk², Gay M. Crooks³

¹Department of Pediatrics and Pharmacology,Pennsylvania State College of Medicine, ²Department of Pharmacology,Pennsylvania State College of Medicine, ³Department of Pediatrics,University of California Los Angeles, School of Medicine

Summary

İşte biz bütün fare karaciğer doku sindirim, hücre zenginleştirme ve flow sitometri izolasyonu gerektiren bir süreçten CD133 eksprese karaciğer kök hücre ve kanser kök hücrelerin izolasyonu tarif. Biz gelişmiş tek hücre izolasyonu ve klonal genişleme için yöntemler içerir.

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CD133+ Liver Stem Cells Clonal Expansion Chronic Liver Injury Hepatocytes Cholangiocytes Liver Cancer Hepatocellular Carcinoma Stem Cell Isolation Solid Organ Rare Population Of Cells Parenchymal Fraction Non-parenchymal Fraction CD45 Negative Cells Enrichment Stem Cell Proliferation Immunohistochemistry Flow Cytometry Core

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Klonal Genişleme CD133 + Karaciğer Kök Hücre İzolasyonu

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Summary

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