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Isolamento de CD133 + células tronco do fígado de Expansão Clonal
JoVE Journal
Biology
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JoVE Journal Biology
Isolation of CD133+ Liver Stem Cells for Clonal Expansion

Isolamento de CD133 + células tronco do fígado de Expansão Clonal

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22,056 Views

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12:06 min

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October 10, 2011

DOI:

10.3791/3183-v

DOI:
10.3791/3183-v

•

12:06 min
October 10, 2011

•

4 Views
, , , ,
1Department of Pediatrics and Pharmacology,Pennsylvania State College of Medicine, 2Department of Pharmacology,Pennsylvania State College of Medicine, 3Department of Pediatrics,University of California Los Angeles, School of Medicine

Summary

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Aqui nós descrevemos o isolamento de células do fígado CD133 expressando-tronco e células-tronco do câncer de fígado murino todo, um processo que requer a digestão dos tecidos, o enriquecimento de células eo isolamento de citometria de fluxo. Nós incluem métodos para o isolamento de células avançado única e expansão clonal.

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Cite this Article

Rountree, C. B., Ding, W., Dang, H., VanKirk, C., Crooks, G. M. Isolation of CD133+ Liver Stem Cells for Clonal Expansion. J. Vis. Exp. (56), e3183, doi:10.3791/3183 (2011).

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