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クローン性増殖のためのCD133 +肝幹細胞の単離
JoVE Journal
Biology
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JoVE Journal Biology
Isolation of CD133+ Liver Stem Cells for Clonal Expansion
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クローン性増殖のためのCD133 +肝幹細胞の単離

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22,056 Views

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12:06 min

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October 10, 2011

DOI:

10.3791/3183-v

DOI:
10.3791/3183-v

•

12:06 min
October 10, 2011

•

4 Views
, , , ,
1Department of Pediatrics and Pharmacology,Pennsylvania State College of Medicine, 2Department of Pharmacology,Pennsylvania State College of Medicine, 3Department of Pediatrics,University of California Los Angeles, School of Medicine

Summary

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Here we describe the isolation of CD133 expressing liver stem cells and cancer stem cells from whole murine liver, a process that requires tissue digestion, cell enrichment, and flow cytometry isolation. We include methods for advanced single cell isolation and clonal expansion.

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CD133+ Liver Stem CellsClonal ExpansionChronic Liver InjuryHepatocytesCholangiocytesLiver CancerHepatocellular CarcinomaStem Cell IsolationSolid OrganRare Population Of CellsParenchymal FractionNon-parenchymal FractionCD45 Negative CellsEnrichmentStem Cell ProliferationImmunohistochemistryFlow Cytometry Core

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Rountree, C. B., Ding, W., Dang, H., VanKirk, C., Crooks, G. M. Isolation of CD133+ Liver Stem Cells for Clonal Expansion. J. Vis. Exp. (56), e3183, doi:10.3791/3183 (2011).

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