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Isolation of CD133+ Liver Stem Cells for Clonal Expansion
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October 10, 2011
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Here we describe the isolation of CD133 expressing liver stem cells and cancer stem cells from whole murine liver, a process that requires tissue digestion, cell enrichment, and flow cytometry isolation. We include methods for advanced single cell isolation and clonal expansion.
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CD133+ Liver Stem CellsClonal ExpansionChronic Liver InjuryHepatocytesCholangiocytesLiver CancerHepatocellular CarcinomaStem Cell IsolationSolid OrganRare Population Of CellsParenchymal FractionNon-parenchymal FractionCD45 Negative CellsEnrichmentStem Cell ProliferationImmunohistochemistryFlow Cytometry Core
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Rountree, C. B., Ding, W., Dang, H., VanKirk, C., Crooks, G. M. Isolation of CD133+ Liver Stem Cells for Clonal Expansion. J. Vis. Exp. (56), e3183, doi:10.3791/3183 (2011).
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