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El aislamiento de islotes humanos de pacientes parcialmente Pancreatectomized
JoVE Journal
Medicine
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JoVE Journal Medicine
Isolation of Human Islets from Partially Pancreatectomized Patients
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El aislamiento de islotes humanos de pacientes parcialmente Pancreatectomized

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15,648 Views

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11:10 min

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July 30, 2011

DOI:

10.3791/2962-v

DOI:
10.3791/2962-v

•

11:10 min
July 30, 2011

•

9 Views
, , , , , , , ,
1Department of GI-, Thorax- and Vascular Surgery,University Hospital Carl Gustav Carus, University of Technology Dresden, 2Molecular Diabetology,Paul Langerhans Institute Dresden, 3Department of Pathology,University Hospital Carl Gustav Carus, University of Technology Dresden

Summary

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La oferta de la diabetes tipo 2 isletas para la investigación es insuficiente. Aquí compartimos nuestro protocolo para el aislamiento de islotes de pacientes sometidos a pancreatectomía parcial. Este enfoque representa un lugar único para la obtención de islotes de diabetes tipo 2 y clínicamente emparejado sujetos no diabéticos en un número suficiente de estudios básicos y clínicos.

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Bötticher, G., Sturm, D., Ehehalt, F., Knoch, K. P., Kersting, S., Grützmann, R., Baretton, G. B., Solimena, M., Saeger, H. D. Isolation of Human Islets from Partially Pancreatectomized Patients. J. Vis. Exp. (53), e2962, doi:10.3791/2962 (2011).

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