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Isolation of Human Islets from Partially Pancreatectomized Patients
JoVE 杂志
医学
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JoVE 杂志 医学
Isolation of Human Islets from Partially Pancreatectomized Patients

Isolation of Human Islets from Partially Pancreatectomized Patients

15,648 Views

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11:10 min

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July 30, 2011

DOI:

10.3791/2962-v

DOI:
10.3791/2962-v

•

11:10 min
July 30, 2011

•

9 Views
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1Department of GI-, Thorax- and Vascular Surgery,University Hospital Carl Gustav Carus, University of Technology Dresden, 2Molecular Diabetology,Paul Langerhans Institute Dresden, 3Department of Pathology,University Hospital Carl Gustav Carus, University of Technology Dresden

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The supply of type 2 diabetic islets for research is insufficient. Here we share our protocol for isolating islets from patients undergoing partial pancreatectomy. This approach represents a unique venue for obtaining islets from type 2 diabetic and clinically matched non-diabetic subjects in adequate numbers for basic and clinical studies.

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Bötticher, G., Sturm, D., Ehehalt, F., Knoch, K. P., Kersting, S., Grützmann, R., Baretton, G. B., Solimena, M., Saeger, H. D. Isolation of Human Islets from Partially Pancreatectomized Patients. J. Vis. Exp. (53), e2962, doi:10.3791/2962 (2011).

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