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Journal / Medicine / Isolation of Human Islets from Partially…
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JoVE Journal Medicine
Isolation of Human Islets from Partially Pancreatectomized Patients
 

Isolation of Human Islets from Partially Pancreatectomized Patients

Article DOI: 10.3791/2962-v • 11:11 min • July 30th, 2011
July 30th, 2011 •
1, 1, 1, 2, 1, 1, 3, 2, 1
1Department of GI-, Thorax- and Vascular Surgery, University Hospital Carl Gustav Carus, University of Technology Dresden, 2Molecular Diabetology, Paul Langerhans Institute Dresden, 3Department of Pathology, University Hospital Carl Gustav Carus, University of Technology Dresden

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The supply of type 2 diabetic islets for research is insufficient. Here we share our protocol for isolating islets from patients undergoing partial pancreatectomy. This approach represents a unique venue for obtaining islets from type 2 diabetic and clinically matched non-diabetic subjects in adequate numbers for basic and clinical studies.

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Human Islets Isolation Partially Pancreatectomized Patients Type 2 Diabetes Islets Of Langerhans Malfunction Limited Availability Organ Donors Pancreatic Tissue Partial Pancreatectomy Pancreatic Diseases Consent Local Ethics Committee Surgical Specimens Soft And Healthy Appearing Pancreatic Tissue Damaged Tissue Diagnostic Purposes 1,000 Islets 2 G Of Pancreatic Tissue Enzyme-containing Media Mince Tissue Digestion Surface Area Ricordi Chamber
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