Journal
/
Medicine
/
Isolamento de Ilhotas Humanos de pacientes parcialmente pancreatectomizado
JoVE Journal
Medicine
Author Produced
This content is Free Access.
JoVE Journal Medicine
Isolation of Human Islets from Partially Pancreatectomized Patients

Isolamento de Ilhotas Humanos de pacientes parcialmente pancreatectomizado

português

Automatically Generated

15,648 Views

•

11:10 min

•

July 30, 2011

DOI:

10.3791/2962-v

DOI:
10.3791/2962-v

•

11:10 min
July 30, 2011

•

9 Views
, , , , , , , ,
1Department of GI-, Thorax- and Vascular Surgery,University Hospital Carl Gustav Carus, University of Technology Dresden, 2Molecular Diabetology,Paul Langerhans Institute Dresden, 3Department of Pathology,University Hospital Carl Gustav Carus, University of Technology Dresden

Summary

Automatically generated

O fornecimento de diabéticos tipo 2 ilhotas para pesquisa é insuficiente. Aqui nós compartilhamos nosso protocolo para isolar ilhotas de pacientes submetidos à pancreatectomia parcial. Esta abordagem representa um local único para a obtenção de ilhotas de diabéticos tipo 2 e clinicamente combinado não-diabéticos em número adequado para estudos básicos e clínicos.

Explore More Videos

Human IsletsIsolationPartially Pancreatectomized PatientsType 2 DiabetesIslets Of Langerhans MalfunctionLimited AvailabilityOrgan DonorsPartial PancreatectomyPancreatic DiseasesConsentLocal Ethics CommitteeSurgical SpecimensDiagnostic Purposes1000 IsletsEnzyme-containing MediaDigestionSurface AreaRicordi Chamber

Read Article

Cite this Article

Bötticher, G., Sturm, D., Ehehalt, F., Knoch, K. P., Kersting, S., Grützmann, R., Baretton, G. B., Solimena, M., Saeger, H. D. Isolation of Human Islets from Partially Pancreatectomized Patients. J. Vis. Exp. (53), e2962, doi:10.3791/2962 (2011).

Download .ris file

Copy

Share Video

.

Copy