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Immuno-fluorescenza saggio di Leptospiral superficie esposti Proteine
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10:47 min
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July 01, 2011
DOI:
1Department of Medicine, David Geffen School of Medicine,University of California, Los Angeles, 2Research service, 151,Veterans Affairs Greater Los Angeles Healthcare System, 3Departments of Medicine, Urology at David Geffen School of Medicine and Department of Microbiology, Immunology and Molecular Gentics,University of California Los Angeles (UCLA), 4Division of Infectious Diseases, 111F,Veterans Affairs Greater Los Angeles Health Care System
Summary
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Un metodo efficace per valutare l'esposizione superficiale di proteine leptospiral è descritto. Il metodo è specificamente progettato per evitare l'interruzione della membrana esterna delle cellule fragili leptospiral. Questa tecnica richiede l'impiego di diversi controlli negativi per valutare l'integrità della membrana esterna e la specificità della reazione anticorpale.
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Cite this Article
Pinne, M., Haake, D. Immuno-fluorescence Assay of Leptospiral Surface-exposed Proteins. J. Vis. Exp. (53), e2805, doi:10.3791/2805 (2011).
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