Journal
/
/
Immuno-fluorescence Assay of Leptospiral Surface-exposed Proteins
Journal JoVE
Immunologie et infection
Un abonnement à JoVE est nécessaire pour voir ce contenu.  Connectez-vous ou commencez votre essai gratuit.
Journal JoVE Immunologie et infection
Immuno-fluorescence Assay of Leptospiral Surface-exposed Proteins

Immuno-fluorescence Assay of Leptospiral Surface-exposed Proteins

15,599 Views

10:47 min

July 01, 2011

DOI:

10:47 min
July 01, 2011

1 Views
,

Summary

Automatically generated

An efficient method to assess surface-exposure of leptospiral proteins is described. The method is specifically designed to avoid disruption of the fragile outer membrane of leptospiral cells. This technique requires employment of several negative controls to assess the integrity of the outer membrane and specificity of antibody reaction.

Read Article