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10:47 min
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July 01, 2011
DOI:
1Department of Medicine, David Geffen School of Medicine,University of California, Los Angeles, 2Research service, 151,Veterans Affairs Greater Los Angeles Healthcare System, 3Departments of Medicine, Urology at David Geffen School of Medicine and Department of Microbiology, Immunology and Molecular Gentics,University of California Los Angeles (UCLA), 4Division of Infectious Diseases, 111F,Veterans Affairs Greater Los Angeles Health Care System
Summary
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レプトスピラタンパク質の表面露出を評価するために効率的な方法が記載されている。方法は、特にレプトスピラ細胞の脆弱な外膜の崩壊を回避するために設計されています。この手法にはいくつかのネガティブコントロールの雇用は、外膜と抗体反応の特異性の整合性を評価する必要があります。
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Cite this Article
Pinne, M., Haake, D. Immuno-fluorescence Assay of Leptospiral Surface-exposed Proteins. J. Vis. Exp. (53), e2805, doi:10.3791/2805 (2011).
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