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10:47 min
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July 01, 2011
DOI:
1Department of Medicine, David Geffen School of Medicine,University of California, Los Angeles, 2Research service, 151,Veterans Affairs Greater Los Angeles Healthcare System, 3Departments of Medicine, Urology at David Geffen School of Medicine and Department of Microbiology, Immunology and Molecular Gentics,University of California Los Angeles (UCLA), 4Division of Infectious Diseases, 111F,Veterans Affairs Greater Los Angeles Health Care System
Summary
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وصفت وسيلة فعالة لتقييم التعرض سطح البروتينات البريميات. وهو مصمم خصيصا لطريقة لتجنب اضطراب الغشاء الخارجي للخلايا هشة البريميات. هذا الأسلوب يتطلب العمل من الضوابط سلبية عديدة لتقييم سلامة الغشاء الخارجي ونوعية رد فعل الأجسام المضادة.
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Cite this Article
Pinne, M., Haake, D. Immuno-fluorescence Assay of Leptospiral Surface-exposed Proteins. J. Vis. Exp. (53), e2805, doi:10.3791/2805 (2011).
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