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Neuroscience
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漂白后荧光标记蛋白的荧光恢复(FRAP)在培养海马神经元的树突棘
JoVE Journal
Neuroscience
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JoVE Journal
Neuroscience
Fluorescence Recovery After Photobleaching (FRAP) of Fluorescence Tagged Proteins in Dendritic Spines of Cultured Hippocampal Neurons
Please note that all translations are automatically generated.
Click here for the English version.
漂白后荧光标记蛋白的荧光恢复(FRAP)在培养海马神经元的树突棘
DOI:
10.3791/2568-v
•
06:35 min
•
April 16, 2011
•
Chan-Ying Zheng
,
Ronald S. Petralia
,
Ya-Xian Wang
,
Bechara Kachar
1
National Institute on Deafness and Other Communication Disorders
,
National Institutes of Health, Bethesda
Chapters
00:05
Title
00:43
Introduction
01:15
Neuron Transfection
02:44
FRAP Experiment on a Spine
04:40
Data Analysis
05:28
Representative Results: FRAP Measurements of EGFP Fluorescence in a Spine from a Cultured Hippocampal Neuron
06:13
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
FRAP已被用来量化绿色荧光蛋白(GFP)标记在培养细胞中的蛋白质的流动性。我们研究分析了GFP的荧光漂白后恢复的百分比移动/静止的分数。在这项研究中,FRAP刺海马神经元。
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Fluorescence Recovery After Photobleaching (FRAP)
GFP-tagged Proteins
Dendritic Spines
Cultured Hippocampal Neurons
Mobility
Excitation Laser
Fluorescence Recovery Rate
Protein Turnover Rate
EGFP Vector
Zeiss 710 Confocal Microscope
Photobleaching
Time Lapse Images
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Immobile Fraction
ImageJ Software
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