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Fluorescence and Bioluminescence Imaging of Subcellular Ca2+ in Aged Hippocampal Neurons
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Neuroscience
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JoVE Journal Neuroscience
Fluorescence and Bioluminescence Imaging of Subcellular Ca2+ in Aged Hippocampal Neurons

Fluorescence and Bioluminescence Imaging of Subcellular Ca2+ in Aged Hippocampal Neurons

8,170 Views

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13:24 min

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December 01, 2015

DOI:

10.3791/53330-v

DOI:
10.3791/53330-v

•

13:24 min
December 01, 2015

•

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1Instituto de Biologìa y Genética Molecular,Consejo Superior de Investigaciones Cientìficas, 2Departamento de Bioquìmica y Biologìa Molecular y Fisiologìa,Universidad de Valladolid

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FluorescenceBioluminescenceSubcellular Ca2+Aged Hippocampal NeuronsExcitotoxicityIntracellular Ca2+ HomeostasisFura2AequorinGFPMitochondrial Ca2+ ConcentrationNMDANeuron Cell DeathNeurodegenerationIschemiaAging

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Calvo-Rodríguez, M., Villalobos, C., Nuñez, L. Fluorescence and Bioluminescence Imaging of Subcellular Ca2+ in Aged Hippocampal Neurons. J. Vis. Exp. (106), e53330, doi:10.3791/53330 (2015).

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