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Fluorescence Recovery After Photobleaching (FRAP) of Fluorescence Tagged Proteins in Dendritic Spines of Cultured Hippocampal Neurons
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Fluorescence Recovery After Photobleaching (FRAP) of Fluorescence Tagged Proteins in Dendritic Spines of Cultured Hippocampal Neurons
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April 16, 2011
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FRAP has been used to quantify the mobility of Green Fluorescence Protein (GFP)-tagged proteins in cultured cells. We examined the mobile/immobile fractions of the GFP by analyzing the fluorescence recovery percentage after photobleaching. In this study, FRAP was performed at spines of hippocampal neurons.
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Fluorescence Recovery After Photobleaching (FRAP)GFP-tagged ProteinsDendritic SpinesCultured Hippocampal NeuronsMobilityExcitation LaserFluorescence Recovery RateProtein Turnover RateEGFP VectorZeiss 710 Confocal MicroscopePhotobleachingTime Lapse ImagesMobile FractionImmobile FractionImageJ SoftwareGraphpad Software
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Zheng, C., Petralia, R. S., Wang, Y., Kachar, B. Fluorescence Recovery After Photobleaching (FRAP) of Fluorescence Tagged Proteins in Dendritic Spines of Cultured Hippocampal Neurons. J. Vis. Exp. (50), e2568, doi:10.3791/2568 (2011).
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