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Bryostatin 1/Ionomycin Yoluyla Tümör-reaktif T hücrelerinin ex vivo Genişleme ve Ortak Gama Zinciri Sitokinler Formülasyon
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Immunology and Infection
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JoVE Journal Immunology and Infection
Ex vivo Expansion of Tumor-reactive T Cells by Means of Bryostatin 1/Ionomycin and the Common Gamma Chain Cytokines Formulation
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Bryostatin 1/Ionomycin Yoluyla Tümör-reaktif T hücrelerinin ex vivo Genişleme ve Ortak Gama Zinciri Sitokinler Formülasyon

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07:20 min

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January 14, 2011

DOI:

10.3791/2381-v

DOI:
10.3791/2381-v

•

07:20 min
January 14, 2011

•

1 Views
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1Department of Microbiology & Immunology,Virginia Commonwealth University- Massey Cancer Center, 2Department of Internal Medicine,Virginia Commonwealth University- Massey Cancer Center, 3Department of Surgery,Virginia Commonwealth University- Massey Cancer Center

Summary

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Için verimli bir protokol<em> Ex vivo</em> Tümör-reaktif T hücrelerinin tümör drenaj lenf düğümlerine veya diğer ikincil lenfoid dokularda tümör taşıyan ana genişleme açıklanmıştır. Bu protokol, meme kanseri evlatlık immünoterapi kullanmak için seçici, tümör spesifik T hücrelerinin genişletir.

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Kmieciak, M., Toor, A., Graham, L., Bear, H. D., Manjili, M. H. Ex vivo Expansion of Tumor-reactive T Cells by Means of Bryostatin 1/Ionomycin and the Common Gamma Chain Cytokines Formulation. J. Vis. Exp. (47), e2381, doi:10.3791/2381 (2011).

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