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ブリオスタチンの1/Ionomycinによる腫瘍反応性T細胞のex vivoでの拡張と共通γ鎖のサイトカインの策定
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Immunology and Infection
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JoVE Journal Immunology and Infection
Ex vivo Expansion of Tumor-reactive T Cells by Means of Bryostatin 1/Ionomycin and the Common Gamma Chain Cytokines Formulation
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ブリオスタチンの1/Ionomycinによる腫瘍反応性T細胞のex vivoでの拡張と共通γ鎖のサイトカインの策定

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14,615 Views

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07:20 min

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January 14, 2011

DOI:

10.3791/2381-v

DOI:
10.3791/2381-v

•

07:20 min
January 14, 2011

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, , , ,
1Department of Microbiology & Immunology,Virginia Commonwealth University- Massey Cancer Center, 2Department of Internal Medicine,Virginia Commonwealth University- Massey Cancer Center, 3Department of Surgery,Virginia Commonwealth University- Massey Cancer Center

Summary

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のための効率的なプロトコル<em>生体外で</em腫瘍所属リンパ節または腫瘍を持つホストの他の二次リンパ組織から腫瘍反応性T細胞の>拡張が記述されています。このプロトコルは、選択的に乳がんの養子免疫療法で使用するための腫瘍特異的T細胞を拡大する。

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Cite this Article

Kmieciak, M., Toor, A., Graham, L., Bear, H. D., Manjili, M. H. Ex vivo Expansion of Tumor-reactive T Cells by Means of Bryostatin 1/Ionomycin and the Common Gamma Chain Cytokines Formulation. J. Vis. Exp. (47), e2381, doi:10.3791/2381 (2011).

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